sgrna scaffold (Addgene inc)
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Addgene inc
sgrna scaffold
Sgrna Scaffold, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 449 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sgrna+cloning+vector/gRNA_Cloning+Vector+(Plasmid+%2341824)/pmc12634846-215-15-21
Average 96 stars, based on 449 article reviews
Sgrna Scaffold, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 449 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sgrna+cloning+vector/gRNA_Cloning+Vector+(Plasmid+%2341824)/pmc12634846-215-15-21
Average 96 stars, based on 449 article reviews
sgrna scaffold - by Bioz Stars,
2026-09
96/100 stars
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1) Product Images from "Loop engineering of AtCas9 for effective and broad genome editing"
Article Title: Loop engineering of AtCas9 for effective and broad genome editing
Journal: Cell Insight
doi: 10.1016/j.cellin.2025.100286
Figure Legend Snippet: Z7 enhances DNA binding and cleavage activity of AtCas9 at low magnesium condition. (a) In vitro cleavage assay of WT and Z7 proteins with different concentrations of Mg 2+ (0.5, 1, 2, 5 mM). Three target loci ( Spacer-21a , CTNNB1-10g , and VEGFA-11g ) were tested. (b) Temperature-dependent cleavage efficiency of WT and Z7. (c) Thermal shift analysis of melting temperatures of the AtCas9 proteins. (d-e) EMSA analysis of binding affinities between Cas9 variants and sgRNA (d), or Cas9 RNP complexes with DNA substrates (e), under the indicated magnesium concentrations.
Techniques Used: Binding Assay, Activity Assay, In Vitro, Cleavage Assay
Figure Legend Snippet: Structure-guided protein engineering improves the editing efficiency of AtCas9 . (a) Schematic representation of sgRNA: DNA target recognition by AtCas9 variants. Residue substitutions in the E535 and E5116 variants are highlighted. (b) Adenine base editing efficiencies of AtCas9 mutants at C-myc-41g and CTNNB1-10g target sites. (c) Solvent-accessible surface area (SASA) analysis of interactions between different AtCas9 variants and nucleic acids, reflecting changes in protein–nucleic acid interface accessibility. (d) (left) A-to-G editing efficiencies of wildtype and AtCas9 E5116 mutant. (right) Median fold change of editing efficiency. Data were normalized to WT AtCas9-ABE. For (b) and (d), data are mean ± SD of n = 3 biologically independent experiments. Statistical significance was determined by unpaired t -test (∗ p < 0.033, ∗∗∗ p < 0.001). Each dot represents one biological experiment.
Techniques Used: Drug discovery, Residue, Solvent, Mutagenesis
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